Automated Determination of Serum 1-Antitrypsin by Antitryptic Activity Measurement
نویسندگان
چکیده
BACKGROUND: 1-Antitrypsin (A1AT) deficiency is currently detectable by protein immunoassay, phenotyping, and genotyping of the S and Z mutations, but no fully automated method for standard biochemical analyzers is yet available. Here, we present a method that measures the antitryptic activity in serum. This method is rapid, automated, and allows the easy evaluation of a large cohort of patients.
منابع مشابه
Automated determination of serum alpha1-antitrypsin by antitryptic activity measurement.
BACKGROUND Alpha1-Antitrypsin (A1AT) deficiency is currently detectable by protein immunoassay, phenotyping, and genotyping of the S and Z mutations, but no fully automated method for standard biochemical analyzers is yet available. Here, we present a method that measures the antitryptic activity in serum. This method is rapid, automated, and allows the easy evaluation of a large cohort of pati...
متن کاملMeasurement of antitryptic activity of serum with a centrifugal analyzer.
The Selected Method [Clin. Chem. 20, 396 (1974)] for the enzymatic assay of alpha1-antitrypsin in serum has been adapted for use with the E.N.I.-GEMSAEC. With alpha-N-benzoyl-D,L-arginine-p-nitroanilide as substrate, the difference between the tryptic activity measured with and without addition of serum in the same run has been used to calculate the trypsin-inhibitory capacity. The rate of incr...
متن کاملCharacteristics of the antitrypsin activity of human serum 1
SYNOPSIS The antitrypsin activity of human serum was studied as a function ofpH, preincubation of serum with trypsin, and the concentrations of substrate, trypsin, and serum. Maximal activity was observed between pH 8-0 and 8-3. Activity was enhanced by preincubation of serum with trypsin, and reduced by high concentrations of trypsin and substrate. Percentage inhibition was a sigmoid function ...
متن کاملCharacteristics of the antitrypsin activity of human serum.
The antitrypsin activity of human serum was studied as a function of pH, preincubation of serum with trypsin, and the concentrations of substrate, trypsin, and serum. Maximal activity was observed between pH 8.0 and 8.3. Activity was enhanced by preincubation of serum with trypsin, and reduced by high concentrations of trypsin and substrate. Percentage inhibition was a sigmoid function of log s...
متن کاملDetermination of alpha-1 antitrypsin by a nephelometric procedure.
Several reports exist which link low levels of serum alpha-1 antitrypsin with pulmonary emphysema. 3 ’9 ’13 Deficiency of this protein may be detected by serum electrophoresis or specifically quantitated by radial immunodiffusion. Recent investigations have led to the development of a number of automated and manual procedures for the quantitation of immunochemical reactions in aqueous media thr...
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